Clean air conditioning system is the key to the safe operation of biosafety laboratories. The biological safety laboratory operation object is hazardous to the human body, microorganisms, animals and plants or the environment is harmful, we must regulate the system through the use of reasonable air, maintain a relatively negative environment of biological safety laboratory whenever it is not destroyed, in order to ensure laboratory dangerous microorganisms can not spread to the external environment.
Super clean bench / biosafety cabinet
Cell cultures are very demanding for sterile environments and are usually performed under 100 levels of air quality during cellular operations, so the ultra clean bench or biosafety cabinet must be equipped.
CO2 incubator is an essential instrument for cell culture. It provides a comfortable environment for cells in vitro, such as suitable temperature, humidity, pH, O2 concentration and so on. When cells are cultured in vitro, they are susceptible to infection by a variety of bacteria and microorganisms. Therefore, the ability of sterilization and bacteriostasis of CO2 incubator is particularly important.
The cell cryopreservation tube from liquid nitrogen or ultra low temperature refrigerator needs to be placed in the water bath at 37 DEG C for a quick recovery and then subcultured. Therefore, the constant temperature water bath is also a necessary instrument in the cell laboratory.
Ultra low temperature freezer
细胞的冻存过程需要一系列程序降温，一般4℃下存放30 min，转放-20℃ 1 hour，再转入-70至-80℃过夜，之后才可以转移到液氮内(-196℃)，这时就必须配备一台-86℃超低温冰箱。一般超低温冰箱保存细胞可达数月，对于不需要长期冻存的细胞，可暂时放于超低温冰箱保存。
The cryopreservation process requires a series of programmed cooling cells, usually under 4 DEG C for 30 min, a -20 C 1 hour, and then transferred to the overnight -70 to -80 DEG C, then can be transferred to liquid nitrogen (-196 C), then it must be equipped with a -86 ultra low temperature refrigerator temperature. In the ultra low temperature freezer, cells can be preserved for several months. For cells that do not need long-term cryopreservation, they can be stored in a cryogenic refrigerator for the time being.
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