1 sampling conditions
Clean air conditioning system in the open state, the concentration of bacteria should be detected in other projects, the whole room surface after routine disinfection. When the air inlet is arranged centrally, the operation area and the surrounding area should be detected respectively. When the air inlet is dispersed, the whole room is unified. The principle of measuring point arrangement in centralized air condition, where all operation room with 3 point pattern; I operation room auxiliary area with 8 point pattern that each side in 2 points; 2 operation room auxiliary zone 6. Each long side pattern in 2 points; grade 4 operation room auxiliary area pattern. When the air outlet is arranged in a decentralized way, the whole room is uniformly distributed, and the measuring points can be evenly distributed, but should not be arranged below the air outlet.
2 sampling method
When the concentration of planktonic bacteria was determined by floatation method. The measured points should be the same as those of the measured area, and the sampling time should not exceed 30 min. When the sedimentation method was used to determine the concentration of settling bacteria, the measured points were not only less than the measured concentration of dust in the measured area, but also meet the requirements of the minimum number of dishes. Sampling must have 2 blank control. The first test was done with a Petri dish for each batch. The second time was in the detection of 1 controls per room or area.
3 sampling height:
Sampling points can be placed on the ground or not higher than the ground 0.8m arbitrary height in the operation area should be no operating table when the operating table has been fixed when the detection height should be above the table 0.25cm.
4 sampling notes
Place the sampling plate from the top area of the total area. Each room is placed in the center of the room. Near the last door arrangement; charge plate in reverse order, from the outside of the room began to close, each room began to collect from the most close to the door, the first layout of the dish and finally closed in the clean room is uniform;; negative control panel. Note the laminar flow clean room should be avoided just below the efficient delivery outlet, if there is a fixed equipment such as surgical bed, can be placed on the device.